The temperature is regulated at 22±1 °C, with a relative humidity (RH) of 55±10% and air exchange rate is set not to fall below 15 air changes/hr. throughout each 24-hour period. The temperature RH and air exchange rate in each room are monitored and recorded in real time by a computerized monitoring system. Sound level and light intensity is not exceed 85 dB, 325 lux respectively in animal housing area. Ammonia levels should not exceed 25 ppm in any type of animal facility.
Food, drinking water, bedding and nesting are used for all rodents in the LAC. Quality control is scheduled for monitoring both nutritional standard values and contamination by in house or outsource laboratories. The contamination monitoring include microorganism, aflatoxin, pesticides, heavy metals and others that related to specific protocol.
Micro- and Macro-Environments
Cages and lids, filtered top cages, ventilated cages, water bottles, racks/shelves, laminar flow racks, safety cabinets and other animal equipment are routine sanitized in scheduled frequency. The effectiveness of sanitization procedures is monitored (e.g., water temperature monitoring, sterility test, microbiological monitoring, visual inspections and other indicators) Animal rooms sanitation is monitored using air sampler both during and after animal housing.