Dr. Nisanart Charoenlap

02 Feb 2023

Name: Dr. Nisanart Charoenlap

Office address: Research Laboratory of Biotechnology, Chulabhorn Research Institute,
54 Kamphaeng Phet 6 Talat Bang Khen, Lak Si, Bangkok 10210, Thailand

Tel: +662-5538555 ext. 3256 (office)

Positions: Senior Research Scientist 2, Research Laboratory of Biotechnology, Chulabhorn Research Institute

E-mail:  [email protected]

Research Interests

  1. Microbioloy
  2. Bacterial genetics
  3. Biotechnology
  4. Antimicrobial resistance (AMR)
  5. Environmental pollutants on AMR

Education and Degrees

  • Mahidol University, Bangkok
    – Ph.D. in Biotechnology, 2007-2009 (Royal Golden Jubilee Scholarship funded by Thailand Research Fund)
    – M.Sc. in Biotechnology, 2002-2006 (Graduate studies Scholarship funded by Ministry of University Affair and Faculty of Graduate studies, Mahidol University)
    – B.Sc. in Biotechnology, 1999-2002 (Sritrangtong Scholarship funded by Faculty of Science, Mahidol University)

Working Experiences

  • Visiting Ph.D. student at Department of Food Sciences, University of California, Davis, USA (UC, Davis) (under supervision of Prof. Chester W. PriceS.A., 2004)
  • Post doctoral Fellow at Department of Biological Engineering, Massachusetts Institute of Technology (MIT), S.A., 2007-2009 (with Prof. David B. Schauer)
  • Project Manager for One Health Drivers of Antibacterial Resistance in Thailand (OH-DART) funded by Medical Research Council (MRC), UK, 2018-2022

Academic Positions

  • Research Scientist, Research Laboratory of Biotechnology, 2009-2015
  • Research Scientist I, Research Laboratory of Biotechnology, 2016-2022
  • Research Scientist II, Research Laboratory of Biotechnology, 2022-present


  1. Charoenlap, N., Dharmsthiti, S., Sirisansaneeyakul, S., and Lertsiri, S. Optimization of cyclodextrin production from sago starch. Bioresour Technol. 2004; 92: 49-54.
  2. Sukchawalit, R., Prapagdee, B., Charoenlap, N., Vattanaviboon, P. and Mongkolsuk S. Protection of Xanthomonas against arsenic toxicity involves the peroxide-sensing transcription regulator OxyR. Res Microbiol. 2005;156:30–4.
  3. Charoenlap, N., Eiamphungporn, W., Chauvatcharin, N., Utamapongchai, S. Vattanaviboon, P. and Mongkolsuk S. OxyR mediated compensatory expression between ahpC and katA and the significance of ahpC in protection from hydrogen peroxide in Xanthomonas campestris. FEMS Microbiol Lett. 2005; 249: 73-8.
  4. Eiamphungporn, W., Charoenlap, N., Vattanaviboon, P. and Mongkolsuk, S. Agrobacterium tumefaciens soxR is involved in superoxide stress protection and also directly regulates superoxide-inducible expression of itself and a target gene. J Bacteriol. 2006;188: 8669-73.
  5. Nawapan, S., Charoenlap, N., Charoenwuttitam, A., Saenkham, P., Mongkolsuk, S., Vattanaviboon, P.  Functional and expression analyses of the cop operon, required for copper resistance in Agrobacterium tumefaciens. J. Bacteriol. 2009;191: 5159-68.
  6. Patikarnmonthon, N.,Nawapan, S, Buranajitpakorn, S., Charoenlap, N., Mongkolsuk, S., Vattanaviboon, P. Copper ions potentiate organic hydroperoxide and hydrogen peroxide toxicity through different mechanisms in Xanthomonas campestris pv. campestris. FEMS Microbiol Lett. 2010; 313(1):75-80.
  7. Buranajitpakorn, S., Piwkam, A., Charoenlap, N., Vattanaviboon, P., and Mongkolsuk, S. Genes for hydrogen peroxide detoxification and adaptation contribute to protection against heat stress in Xanthomonas campestris pv. campestris. FEMS Microbiol Lett. 2011;317(1):60-6.
  8. Charoenlap, N., Buranajitpakorn, S., Daung-nkern, J., Namchaiw, P., Vattanaviboon, P. and Mongkolsuk S. Evaluation of the virulence of Xanthomonas campestris pv. campestris mutant strains that lacking functional genes in the OxyR regulon. Curr Microbiol. 2011;63(2):232-7.
  9. Mahavihakanont, A., Charoenlap, N., Namchaiw, P., Eiamphungporn, W., Chattrakarn, S., Vattanaviboon, P., Mongkolsuk, S. Novel roles of SoxR, a transcriptional regulator from Xanthomonas campestris, in sensing redox-cycling drugs and regulating a protective gene that have overall implications for bacterial stress physiology and virulence on a host plant. J Bacteriol. 2012;194(2):209-17.
  10. Charoenlap, N., Shen Z., McBee ME., Muthupalani S., Wogan GN., Fox JG., Schauer DB. Alkyl hydroperoxide reductase is required for Helicobacter cinaedi  intestinal colonization and survival under oxidative stresses in BALB/c and BALB/c IL10-/- mice. Infect. Immun. 2012;80(3):921-8.
  11. Leesukon, P. Wirathorn, W., Chuchue, T. Charoenlap, N. and Mongkolsuk, S. The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat. Arch Microbiol. 2013;195(9):671-4.
  12. Sornchuer, P., Namchaiw, P., Kerdwong, J., Charoenlap, N., Mongkolsuk, S., Vattanaviboon, P. Copper chloride induces antioxidant gene expression but reduces ability to mediate H2O2 toxicity in Xanthomonas campestris. Microbiology.2014;160(Pt 2):458-66.
  13. Charoenlap, N., Sornchuer, P., Piwkam, A., Srijaruskul, K., Mongkolsuk, S., Vattanaviboon P. The roles of peroxide protective regulons in protecting Xanthomonas campestris pv. campestris from sodium hypochlorite stress. Can J Microbiol. 2015 May;61(5):343-50. doi: 10.1139/cjm-2014-0792. Epub 2015 Mar 6.
  14. Srijaruskul, K., Charoenlap, N., Namchaiw, P., Chattrakarn, S., Giengkam, S., Mongkolsuk, S., Vattanaviboon, P. Regulation by SoxR of mfsA, Which Encodes a Major Facilitator Protein Involved in Paraquat Resistance in Stenotrophomonas maltophilia. PLoS One. 2015 Apr 27;10(4):e0123699. doi: 10.1371/journal.pone.0123699. eCollection 2015.
  15. Dulyayangkul, P., Charoenlap, N., Srijaruskul, K., Mongkolsuk, S., Vattanaviboon,.P. Major facilitator superfamily MfsA contributes to multidrug resistance in emerging nosocomial pathogen Stenotrophomonas maltophilia. J Antimicrob Chemother. 2016 Jun 21. Doi:10.1093/jac/dkw233
  16. Rittiroongrad, S., Charoenlap, N., Giengkam, S., Vattanaviboon, P., Mongkolsuk, S. Agrobacterium tumefaciens estC, encoding an Eenzyme containing esterase activity, is regulated by EstR, a regulator in the MarR family. PLoS One. 2016 Dec 30;11(12):e0168791. doi: 10.1371/journal.pone.0168791. eCollection 2016.
  17.  Chanhom, P., Charoenlap, N., Tomapatanaget, B., Insin, N. Colloidal titania-silica-iron oxide nanocomposites and the effect from silica thickness on the photocatalytic and bactericidal activities. JMM. 2017(427);60-66.
  18. Panmanee, W., Charoenlap, N., Atichartpongkul, S., Mahavihakanont, A., Whiteside, MD., Winsor, G., Brinkman, FSL., Mongkolsuk, S., Hassett, DJ. The OxyR-regulated phnW gene encoding 2-aminoethylphosphonate:pyruvate aminotransferase helps protect Pseudomonas aeruginosa from tert-butyl hydroperoxide. PLoS One. 2017 Dec 7;12(12):e0189066. doi: 10.1371/journal.pone.0189066. eCollection 2017.
  19. Vattanaviboon, P., Dulyayangkul, P., Mongkolsuk, S., Charoenlap, N.  Overexpression of Stenotrophomonas maltophilia major facilitator superfamily protein MfsA increases resistance to fluoroquinolone antibiotics. J Antimicrob Chemother. 2018(73);1263-66.
  20. Chanhom, P., Charoenlap, N., Manipuntee, C., Insin, N. Metalloporphyrins-sensitized titania-silica-iron oxide nanocomposites with high photocatalytic and bactericidal activities under visible light irradiation. JMM. 2018(475);602-610.
  21. Charoenlap, N., Jiramonai, L., Chittrakanwong, J., Tunsakul, N., Mongkolsuk, S., Vattanaviboon,.P. Inactivation of ahpC renders Stenotrophomonas maltophilia resistant to the disinfectant hydrogen peroxide. Antonie Van Leeuwenhoek. 2019 (112);809-814.
  22. Chittrakanwong, J.*, Charoenlap, N. *, Mongkolsuk, S., and Vattanaviboon, P. mfsQ encoding an MFS efflux pump mediates adaptive protection of Stenotrophomonas maltophilia against benzalkonium chloride. 2020(5);1-5.
  23. Dulyayangkul, P., Satapoomin, N., Avison, M. B., Charoenlap, N., Vattanaviboon, P., and Mongkolsuk, S. Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa. Frontier in Microbiology. 2020(12);11:592153.
  24. Chittrakanwong, J*., Charoenlap, N*, Vanitshavit, V., Sowatad, A., Mongkolsuk, S., Vattanaviboon, P. The role of MfsR, a TetR-type transcriptional regulator, in adaptive protection of Stenotrophomonas maltophilia against benzalkonium chloride via the regulation of mfsQ. FEMS Microbiol Lett. 2021(15);368:fnab098.
  25. Boonyakanog, A*., Charoenlap, N.*, Chattrakarn, S., Vattanaviboon, P., Mongkolsuk, S. Contribution of Stenotrophomonas maltophilia MfsC transporter to protection against diamide and the regulation of its expression by the diamide responsive repressor DitR. PLoS One. 2022;17(8):e0272388.
  26. Chatree, Y., Charoenlap, N.*, Vanitshavit, V., Ruangrassamee, P., Mongkolsuk, S., and Vattanaviboon.  Induction of antimicrobial resistance of Stenotrophomonas maltophilia by exposure to non-lethal levels of antibiotics. Microb. Drug Resist. In press


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