Previous Next Animal research conducted at CRI includes the search for new anti-malarial drugs, and factors affecting susceptibility to malarial infection; studies on animal models of various types of cancers; toxicity of metals, pesticides and air pollutants; acute and chronic toxicity of chemicals found in natural sources; and the health effects of climate change. The major users of the animal facility are researchers from laboratories of Environmental Toxicology, Pharmacology, Immunology, as well as Translational Research Center.

Previous Next 2 Full-time veterinarians (1 Attending Veterinarian and 1 Clinical Veterinarian) has direct responsibility for ensuring adequate veterinary care and is also responsible for ensuring adequate resources to care for animals; proper methods to prevent diseases; communication regarding animal health issues; advising technical and professional staff on proper animal care and use; ensuring proper care and storage of equipment and supplies; and all other aspects of the veterinary care program. Also, the duties engage in protocol support discussion with investigators and coordinates with the veterinary technician. The veterinary technician and animal caretakers are available during the workday from 9 am to 5 pm. They observe the animals during feeding and/or treatment/protocol-related activities. The animals are observed at least once daily also weekend and holiday. After hours observation or treatment is done on an “as needed” basis, when there is a scheduled protocol or emergency medical attention is required.

  Guideline     Guide for the care and use of Laboratory Animals (EN) Guide for the care and use of Laboratory Animals (TH) Occupational Health the Care and Use AVMA Guidelines For Euthanasia   Document   จรรยาบรรณการดำเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ พศ2559 Ethical Principles and Guidelines for the Use of Animals พระราชบัญญัติสัตว์เพื่องานทางวิทยาศาสตร์ พ.ศ. 2558 (ฉบับภาษาไทย) พระราชบัญญัติสัตว์เพื่องานทางวิทยาศาสตร์ พ.ศ. 2558 (ฉบับภาษาอังกฤษ) จรรยาบรรณนักวิจัย Researcher’s Code of Ethics ข้อกําหนดจรรยาบรรณการดําเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ ข้อกําหนดจรรยาบรรณการดําเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ (ฉบับที่2) พ.ศ.2560 Link 1.The Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) 2.Thai Association for Laboratory Animal Science (TALAS) 3.สถาบันพัฒนาการดำเนินการต่อสัตว์เพื่องานทางวิทยาศาสตร์ (สพสว.) 4.American Association for Laboratory Animal Science learning library (AALAS learning library)

All personnel working with animals are trained prior to commencing work. The LAC provides in-house training on • ethics and welfare in animal experimentation • protocol writing • animal handling, basic techniques and related procedures to investigators and technical staff • on the job training in animal husbandry • standard operating procedures (SOPs) related to individual task • specific procedure training programs as requested by investigators       CRI’s Occupational Health and Safety (OHS) Office provides annual safety training for CRI employees includes topics such as: General Laboratory Safety and Lab Waste Management, General Biosafety and Blood-borne Pathogens, Chemical Safety, Fire Prevention-Emergency Preparedness and Rescue Plan.      

Animal Environment       The temperature is regulated at 22±1 °C, with a relative humidity (RH) of 55±10% and air exchange rate is set not to fall below 15 air changes/hr. throughout each 24-hour period. The temperature RH and air exchange rate in each room are monitored and recorded in real time by a computerized monitoring system. Sound level and light intensity is not exceed 85 dB, 325 lux respectively in animal housing area. Ammonia levels should not exceed 25 ppm in any type of animal facility. Animal Supplies       Food, drinking water, bedding and nesting are used for all rodents in the LAC. Quality control is scheduled for monitoring both nutritional standard values and contamination by in house or outsource laboratories. The contamination monitoring include microorganism, aflatoxin, pesticides, heavy metals and others that related to specific protocol. Micro- and Macro-Environments       Cages and lids, filtered top cages, ventilated cages, water bottles, racks/shelves, laminar flow racks, safety cabinets and other animal equipment are routine sanitized in scheduled frequency. The effectiveness of sanitization procedures is monitored (e.g., water temperature monitoring, sterility test, microbiological monitoring, visual inspections and other indicators) Animal rooms sanitation is monitored using air sampler both during and after animal housing.    

Animal research conducted at CRI includes the search for new anti-malarial drugs, and factors affecting susceptibility to malarial infection; studies on animal models of various types of cancers; toxicity of metals, pesticides and air pollutants; acute and chronic toxicity of chemicals found in natural sources; and the health effects of climate change. The major users of the animal facility are researchers from laboratories of Environmental Toxicology, Pharmacology, Immunology, as well as Translational Research Center.  

2 Full-time veterinarians (1 Attending Veterinarian and 1 Clinical Veterinarian) has direct responsibility for ensuring adequate veterinary care and is also responsible for ensuring adequate resources to care for animals; proper methods to prevent diseases; communication regarding animal health issues; advising technical and professional staff on proper animal care and use; ensuring proper care and storage of equipment and supplies; and all other aspects of the veterinary care program. Also, the duties engage in protocol support discussion with investigators and coordinates with the veterinary technician. The veterinary technician and animal caretakers are available during the workday from 9 am to 5 pm. They observe the animals during feeding and/or treatment/protocol-related activities. The animals are observed at least once daily. After hours observation or treatment is done on an “as needed” basis, when there is a scheduled protocol or emergency medical attention is required.

CRI-LAC Organization Chart CRI Organization Chart

Previous Next The animal facility is located at the Chulabhorn Research Institute. The facility is housed in one separate 5-storey building that is connected to the Biomedical Research Building on the Mezzanine and 2nd floors at the ends of 2 wings. The total area is approximately 2,660 square meters. The animal facility is a clean conventional facility. There are separate corridors designated as clean or dirty, and a 1-way workflow through the animal rooms. The ground floor is an open lobby external to the building. The Mezzanine floor is a support area for the building that contains the cage wash area, feed and bedding storage areas, drinking water preparation area, animal receiving room, and laundry area. It also houses the air handling units that serve the Mezzanine floor. The second floor is for general animal research. The third floor is non- clinical GLP study facility. The fourth floor is for inhalation toxicology. The fifth floor houses the air handling units that serve the second, third and fourth floors. Access to the building is restricted to only those who are conducting work in the LAC, which includes LAC personnel, researchers and maintenance personnel.

The CRI Laboratory Animal Center (LAC) has primary responsibility for the day-to-day management of the research animal facility, as well as for implementation of CRI’s ACUP. It is headed by the Director of LAC, a qualified veterinarian with a wealth of experience in care and management of laboratory animal breeding and laboratory animal medicine, and staffed with full-time attending veterinarian, clinical veterinarian, facility engineer, animal technician and animal care workers, all of whom have been appropriately trained in their areas of responsibility and who undergo continual training as necessary according to CRI policy. MISSION Laboratory animal management and husbandry Veterinary preventive medicine and medical care Consultation pertaining to animal care and use, as well as veterinary medical issues in animal use protocol development Support for compliance with animal use protocols carried out in its facilities Training in the care and use of laboratory animals Reporting of requirements to the Institute Animal Care and Use Committee (IACUC)

Three international Courses on Occupational Health and Safety and Environmental Health are planned for summer 2017: “Global Occupational and Environmental Determinants of Diseases: a multidisciplinary approach for prevention“, July 10-14, at the University of Brescia, Italy; “Advanced International Training Course in Occupational and Environmental Health“, July 17-21, at the Chulabhorn Research Institute in Bangkok, Thailand; “Teaching interventions crossing borders“, August 21-September 1, at the Ludwig Maximillian University of Munich, Germany. In addition to high-quality, hands-on training in occupational health and safety, these courses provide unique opportunities for networking among OEH professionals from both high-income countries and low- and middle-income countries and for developing multi-national approaches to continuing education that extend beyond the courses. Students and faculty attending the three courses are encouraged to provide information about occupational and environmental health in their countries including such aspects as OHS services coverage and organization; workers’ benefits and compensation; ratification and implementation of ILO and WHO policies; preventive interventions in environmental health; and remediation of hazardous waste sites. To provide an opportunity for young OEH professionals to publish their work in a peer-reviewed journal, students and faculty participating in the courses are invited to submit publications in a special issue of the Annals of Global Health that will be specifically dedicated to Occupational and Environmental Global Health and guest edited by the Directors...

Development of a GNA-lectin based immunochromatographic test for rapid diagnosis in human penicilliosis marneffeiPenicillium marneffei is classified as an important emerging opportunistic fungal infection. It has been recently renamed as Talaromyces marneffei. It is the most prevalent systemic mycotic infection in patients infected with human immunodeficiency virus (HIV). This infection is endemic in tropical Asia including Thailand, northeastern India, southern China, Hong Kong, Vietnam and Taiwan. The microorganism is the only known temperature-dependent dimorphic fungus of the genus Penicillium.In the purpose of this study, a novel sandwich ICT for screening P. marneffei antigen in urine and/or serum samples will be developed. The monoclonal anti-P. marneffei antigen gold conjugate will be used as specific signal developing agent and Galanthus nivalis agglutinin (GNA), (or polyclonal anti- P. marneffei antigen) immobilized at test line zone will be used as capturing molecule acting as polyclonal anti-terminal mannose. The diagnostic performance of the novel sandwich ICT strip will be evaluated in comparison to that of the hemoculture-confirmed penicilliosis marneffei which is used as the gold standard laboratory diagnosis of penicilliosis marneffei.